Journal: Psychiatry investigation

Article Title: Memory Decline and Aberration of Synaptic Proteins in X-Linked Moesin Knockout Male Mice.

doi: 10.30773/pi.2024.0186

Figure Lengend Snippet: Figure 7. Risperidone reversed behavioral and molecular deficits in moesin-KO. Risperidone was administered for 2 weeks and validated by behavioral experiments at 2 months of age. A: Risperidone reversed memory impairment in moesin-KO in the passive avoidance test and (B) total entry number (left panel) and spontaneous alternation percentage (right panel) in the Y maze. C: Risperidone induced the expression of PSD95, the phosphorylation of synapsin at Ser605 and Ser549, and ERK phosphorylation in the cortex of moesin-KO, and the data are dis played in the graph. All data were analyzed by one-way ANOVA and are presented as the mean±SEM. *p<0.05; **p<0.01; ***p<0.001. KO, knockout mice; WT, wild-type mice; ERK, extracellular signal-regulated kinase; SEM, standard error of the mean; Ris, risperidone.

Article Snippet: The tissue lysates (30 μg) were electrophoresed and transferred to 0.45 μm nitrocellulose membranes, which were incubated with anti-GFAP (ab7260, 1:1,000, Abcam, Cambridge, MA, USA), anti-MAP2 (ab5392, 1:1,000, Abcam), anti-Sox2 (#4900, CST, 1:1,000), anti-DCX (#91954, CST, 1:1,000), anti-soluble NSF adapter protein 25 (SNAP25) (#5308, CST, 1:1,000), anti-postsynaptic density protein 95 (PSD95) (#3409, CST, 1:1,000), anti-phospho-PSD95 (#45737, CST, 1:1,000), or anti-moesin (#3146, CST, 1:1,000), anti-syntaxin1A (#18572, CST, 1:1,000), anti-synaptophysin (#5467, CST, 1:1,000), anti-CDK5 (#14145, CST, 1:1,000), anti-radixin (#2636, CST, 1:1,000), anti-synapsin 1 (#2312, CST, 1:1,000), anti-phospho-synapsin 1 (Ser9) (#2311, CST, 1:1,000), anti-phospho-synapsin 1 (Ser605) (#88246, CST, 1:1,000), anti-phospho-synapsin 1 (Ser549) (#PA1-4697, 1:1,000; Thermo Fisher Scientific, Waltham, MA, USA), antiERK (#4695, CST, 1:1,000), anti-phospho-ERK (#4370, CST, 1:1,000), anti-CREB (#9197, CST, 1:1,000), anti-phosphoCREB (#9190, CST, 1:1,000), anti-mammalian uncoordinated18 (MUNC18) (#13414, CST, 1:1,000), anti-phosphoMUNC18 (ab183484, 1:1,000, Abcam) anti-β-actin (#4967, CST, 1:1,000) for 16 hours at 4°C.

Techniques: Expressing, Phospho-proteomics, Knock-Out

Journal: PLoS ONE

Article Title: Cigarette Smoking Accelerated Brain Aging and Induced Pre-Alzheimer-Like Neuropathology in Rats

doi: 10.1371/journal.pone.0036752

Figure Lengend Snippet: The total lysate of the hippocampus of the rats were subjected to Western blotting analysis. The levels of p-ERK1/2, total ERK1/2 (a), p-JNK and total JNK (b), p-GSK3β (Ser9), p-GSK3β (pY216), p-PP2A (pY307) (e) and cleaved caspase-3 (i) were detected. α-Tubulin was used as loading control. Quantitative analysis of the band intensity of the detected kinases was shown in (b, d, f–g, and j). *P<0.05 compared to control.

Article Snippet: Rabbit polyclonal antibodies against phosphorylated ERK (P44/42), total ERK, phosphorylated JNK (Thr183 or Tyr185), total JNK, cleaved caspase-3, phosphorylated GSK-3β (Ser9) and mouse monoclonal antibody against synapsin-1 were from Cell Signaling Technology (Boston, MA, USA).

Techniques: Western Blot, Control